FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33529029
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCCT679100.021.0717328
TGTAGCC645000.021.0448327
CTGTAGC672200.020.9330226
CTAGCTG658750.020.7449342
GCTGTAG656800.020.680455
CCTAGCT713250.020.6328951
TAGCTGT665850.020.5513553
AGCTGTA665800.020.4273364
TAGCCTA681350.020.2062329
ATGCTTC325850.020.1806283
TCTATCG332650.019.7347378
AGCCTAT725450.019.5813810
CTTCTAT329600.019.3667776
GCCTATT195400.019.25269711
TTATGCT391800.019.2465921
ATAGTGA174050.019.2325
TATCGCC341150.019.23013310
GCTTCTA331350.019.2082585
TTCTATC345550.019.1253367
CTATCGC348850.018.8119819
Produced by FastQC (version 0.11.5)