FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33529029
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG504380.1504308400938184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA270050.071.4959269
GGACGTT306100.069.379119
GCACGTT230100.067.8032469
GGCATTA169800.066.402179
GTACGTT234350.065.195369
CGACGTT219350.064.596759
TCACGTT201250.064.218959
TTACGTT208750.062.176369
AGACGTT245750.060.9777649
GGTCCGT188650.060.7810029
ATACGTT208000.060.7455259
TGGTATA211800.060.3780339
TAACGTT226850.060.3253449
ACACGTT201550.059.083759
AGGTATA180900.059.0151869
CGTCCGT121900.058.1185269
CTACGTT181750.058.0844549
TGACGTT261650.057.7108089
GAACGTT288200.057.009949
CCACGTT187050.056.5976949
Produced by FastQC (version 0.11.5)