FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30044677
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG442570.14730396336096407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA241900.071.664819
GGACGTT280950.069.902879
GCACGTT207800.067.288139
CGACGTT194150.066.874729
GGCATTA156550.066.512189
GTACGTT212000.064.1508269
TCACGTT174650.063.3909459
TTACGTT185600.061.8721859
TAACGTT206800.061.3864949
AGACGTT218000.061.2739649
TGGTATA193650.061.0559129
GGTCCGT167000.060.8995829
AGGTATA162200.060.1077779
ATACGTT187300.060.0853089
ACACGTT178700.059.2430349
TTTCTCA111350.059.160199
TGACGTT224500.058.136869
CCACGTT164050.057.9014439
GAACGTT260300.057.259789
CTACGTT159300.057.1201029
Produced by FastQC (version 0.11.5)