FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31893117
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGCC620050.021.1146377
CTGTAGC643500.020.9606386
GTAGCCT654800.020.918038
CTAGCTG632050.020.8153172
GCTGTAG631050.020.7293785
CCTAGCT680400.020.6766241
TAGCTGT639750.020.593663
AGCTGTA639350.020.4757544
ATGCTTC318950.020.182623
TAGCCTA656200.020.1458849
TCTATCG322200.019.8900248
AGCCTAT696300.019.56545410
CTTCTAT319250.019.4019936
TATCGCC332700.019.26560210
TTCTATC332700.019.2556847
TTATGCT376550.019.2429851
GCCTATT181200.019.21347411
ATAGTGA163150.019.1279055
GCTTCTA322300.019.1263875
CTATCGC338850.018.922439
Produced by FastQC (version 0.11.5)