FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31893117
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG467300.14652064268287104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA257950.070.911649
GGACGTT290250.070.0486459
GCACGTT219500.068.347249
CGACGTT209900.066.796039
GGCATTA166350.066.5782559
GTACGTT223000.064.70179
TCACGTT184050.062.9463849
TTACGTT193750.061.9665769
GGTCCGT174850.061.2270479
ATACGTT202650.060.9228679
TGGTATA203850.060.7727139
TAACGTT217650.060.766159
AGACGTT233200.060.3226289
ACACGTT194400.059.289019
AGGTATA175800.059.2523089
CCACGTT183300.058.937799
TTTCTCA121050.058.8774579
CGTCCGT114650.058.4201859
GAACGTT278550.057.7336169
TGACGTT242800.057.6224759
Produced by FastQC (version 0.11.5)