FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48973737
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGCC955500.021.0753867
CTGTAGC987950.020.944376
GTAGCCT1008250.020.9262818
CTAGCTG972350.020.8053592
CCTAGCT1043800.020.7419261
GCTGTAG968650.020.6828465
TAGCTGT985400.020.5478613
AGCTGTA984800.020.4241074
ATGCTTC489300.020.3320663
TAGCCTA1005200.020.2259569
TCTATCG500100.019.822278
AGCCTAT1071600.019.56805210
TATCGCC509300.019.4512410
CTTCTAT498000.019.3735926
TTCTATC517550.019.3303387
TTATGCT584100.019.2780131
ATAGTGA246450.019.1123265
GCCTATT285950.019.1031811
CTATCGC520950.019.0289219
GCTTCTA501450.019.025565
Produced by FastQC (version 0.11.5)