FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52158569
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGCC1013750.021.058027
GTAGCCT1066600.020.9737138
CTGTAGC1051150.020.9399346
CTAGCTG1027150.020.8215222
GCTGTAG1029550.020.675325
CCTAGCT1107950.020.6396181
TAGCTGT1042950.020.5172793
AGCTGTA1041850.020.4460094
ATGCTTC528400.020.2202843
TAGCCTA1067700.020.1876629
TCTATCG536600.019.8761358
AGCCTAT1141000.019.50294910
CTTCTAT531250.019.4738926
TATCGCC547900.019.45616710
TTCTATC553950.019.366797
TTATGCT623300.019.327061
GCTTCTA536350.019.180175
GCCTATT303200.019.04671711
CTATCGC559900.019.041149
ATAGTGA273400.019.0349045
Produced by FastQC (version 0.11.5)