FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39186634
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGCC751800.021.0737197
CTGTAGC776950.020.970676
GTAGCCT792700.020.9549888
CCTAGCT826050.020.666521
CTAGCTG771800.020.649672
GCTGTAG765450.020.6376695
AGCTGTA773900.020.48344
TAGCTGT780050.020.4417723
TAGCCTA790900.020.2432969
ATGCTTC389550.020.2407953
TCTATCG396400.019.832658
AGCCTAT847300.019.53324510
CTTCTAT394100.019.4488266
TATCGCC406250.019.34095210
ATAGTGA202050.019.2887945
TTATGCT463650.019.2320821
TTCTATC411700.019.213177
GCTTCTA396300.019.1771435
CTATCGC414500.018.9613069
GCCTATT220450.018.95114311
Produced by FastQC (version 0.11.5)