FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41639898
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGCC799550.021.0189887
GTAGCCT839400.020.9436868
CTGTAGC828950.020.9118426
CTAGCTG814850.020.7314682
CCTAGCT877900.020.636031
GCTGTAG815900.020.5952365
TAGCTGT822950.020.5297953
AGCTGTA820100.020.4777134
ATGCTTC416050.020.2604163
TAGCCTA844400.020.0966749
TCTATCG426750.019.7548128
AGCCTAT897050.019.56092510
TATCGCC434650.019.38310210
TTATGCT499600.019.285731
CTTCTAT425750.019.2767756
TTCTATC440400.019.2249457
GCTTCTA424700.019.2002015
ATAGTGA218400.019.1644385
GCCTATT234600.019.06471411
CTATCGC444950.018.9517179
Produced by FastQC (version 0.11.5)