FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41639898
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG627810.15077126269617663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA338600.070.702919
GGACGTT390500.069.218249
GCACGTT288500.068.3675849
CGACGTT274050.066.094939
GGCATTA215050.065.730559
GTACGTT297900.064.284349
TCACGTT239100.063.8648879
TTACGTT250300.062.0259329
TGGTATA261400.061.310589
TAACGTT277400.060.9609839
GGTCCGT229000.060.705719
ATACGTT263300.060.287089
AGACGTT305950.059.773059
AGGTATA226550.059.429989
TTTCTCA157800.059.4132089
ACACGTT258550.058.0906879
CCACGTT232400.057.8425649
TGACGTT316750.057.65459
CGTCCGT148700.056.7327739
CTACGTT221100.056.6084259
Produced by FastQC (version 0.11.5)