FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15030501
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10128816.738837248339228No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1466240.9755097318446004No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1121930.7464355313239392No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT622620.4142376890830186No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT445770.2965769404492904No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA421060.2801370360176284No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG349010.23220117546314656No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC281190.1870795923569015No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT235790.15687434504012873No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC232500.15468546258038904No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT205590.13678186775011691No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG205520.1367352957828884No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC202160.13449984135592022No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT192410.12801303163480712No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC165430.11006286483730648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1122500.083.544051
CGTTATC1127750.083.1296162
TTATCCA1175400.079.714584
GTTATCC1213800.077.259243
TATCCAC1217650.077.1615455
ATCCACC1227750.076.682556
CACGCTG146800.074.9277041
TCCACCT1326800.070.800997
CCACCTT1326300.070.76688
CACCTTC1366400.068.7677469
GGAAGTT273550.060.537442
AAGTTTC276100.059.522574
CTCGTAT190150.057.707718
TTCTGGC295050.057.600968
TTTCTGG293250.057.2444047
ACGCTGC195150.056.630012
GCTCGTA194600.056.4972767
TCGTATC194050.056.43849
CTGCTCG196950.056.08215
TGCTCGT202400.054.655976