FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37228820
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCCC661350.021.5092625
TTTCCCT669500.021.364086
CTTTTCC681600.021.2478734
CTAGACC476050.020.9000436
CCCTCAG708150.020.7223019
GATTTCC334800.020.7085362
TCTTTTC703350.020.6826343
TGATTTC399600.020.4454351
TAGACCT493450.020.3740757
GACGGCT569300.020.3290631
AGACCTA508600.020.2732528
CTCTAAA418100.020.2197632
TAAAGGC400650.020.1961125
ATTTCCA343100.020.189593
TCCCTCA727250.020.1780668
GGCGTAC421150.020.0794339
ACCTAGG514350.020.02309210
AGTCTTT782600.019.98631
GCGTACA423850.019.93854710
GCTAGAC510050.019.914455
Produced by FastQC (version 0.11.5)