FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14905537
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCATC276600.019.5335922
TGAGCAT281500.019.5195141
TATGAGA275650.019.42171910
GACCAAT262550.019.4196031
TGGGTGT297100.019.4040813
CATATGA276650.019.380438
CGTAGAA232250.019.379952
ATGACAA170400.019.3485622
CCTCTGA179050.019.3415181
AGGCCAC267500.019.3369771
GCCTCTA234500.019.3323571
CTGAAAC156900.019.3305441
GCCACTC194800.019.3273563
CTGGGTG297900.019.3151782
GTAAACT231700.019.31231510
GACCTGG162850.019.299951
CTGATCC172550.019.299081
GGCCACT195550.019.2789292
TCGTAGA239800.019.2679961
CTGCCTA167750.019.254991