FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37228820
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG547690.14711452041724662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA316650.072.208119
GGACGTT360500.069.248749
GCACGTT259850.068.0229
GGCATTA216650.067.7754759
TCACGTT212100.065.843079
CGACGTT239850.065.7735759
GTACGTT267450.064.833699
TGGTATA247450.064.217189
TAACGTT244050.063.2310879
TTACGTT232500.062.5884259
AGGTATA210000.062.312279
AGACGTT278100.062.1063359
GTGTATA205700.060.825659
TGACGTT276050.060.2736139
CCACGTT214750.060.1028149
ATACGTT237850.060.0013129
GCGTATA184450.059.6996739
ACACGTT234200.059.629879
GAGTATA163800.058.8453759
CTACGTT198550.058.1999369
Produced by FastQC (version 0.11.5)