FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15005265
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGAGA277700.019.45494810
CATATGA278900.019.4214348
ATGACAA175050.019.412242
TGAGCAT285600.019.3919051
GACCAAT259400.019.3801291
AGGCCAC270850.019.3732991
GAGCATC280550.019.3447862
TGGGTGT300250.019.3436833
GCCTCTA235750.019.3386761
CTGATCC181450.019.3322731
CCTCTGA180250.019.3222471
CCGTGAA177300.019.2886966
CTGGGTG301500.019.2775882
CGGCGCT195500.019.2672569
GTAAACT230350.019.26504710
TCGTAGA236150.019.2550931
CCTAGAA160050.019.24279
GCCTACA169550.019.2330683
GCCACTC194200.019.2325533
ATATGAG281450.019.231269