FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37898297
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG564350.14891170439663817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA325750.073.008429
GGACGTT363450.069.025229
GCACGTT264100.067.956729
GGCATTA225850.067.309849
CGACGTT253250.067.125929
TCACGTT215950.065.0622949
GTACGTT271650.064.925929
TGGTATA253450.064.373279
TAACGTT253550.063.9623579
AGGTATA219400.062.915719
TTACGTT239250.062.758339
AGACGTT265450.062.247699
ATACGTT238500.061.0311979
TGACGTT280000.059.9389089
ACACGTT230700.059.926129
GTGTATA208900.059.6696249
GAGTATA162950.059.256189
GCGTATA182450.059.0491689
GAACGTT323900.058.8479359
CTACGTT198200.058.7954259
Produced by FastQC (version 0.11.5)