FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15005265
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9801126.531787342642732No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1370980.9136659699112278No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1086740.7242391254003178No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT573580.38225249604055644No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT417750.27840228079943935No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA403430.26885896383702657No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG325630.2170104959825768No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC263450.17557170766394328No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC215450.1435829357228946No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT211740.14111047022495105No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT199860.1331932491695415No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG196320.13083407723888915No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC185050.12332338016023044No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT177340.11818518366719948No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC156140.10405680939323632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1091050.083.685991
CGTTATC1097950.083.1474152
TTATCCA1145850.079.6376654
GTTATCC1186450.077.105913
TATCCAC1193700.076.911745
ATCCACC1203350.076.4503566
CACGCTG153100.074.265521
CCACCTT1301000.070.574988
TCCACCT1305200.070.399987
CACCTTC1338850.068.6305859
GGAAGTT261900.060.619572
AAGTTTC261550.060.0390054
TTCTGGC287200.058.7462128
TTTCTGG285350.057.7866677
CTCGTAT196250.057.386548
ACGCTGC201550.056.6686172
GCTCGTA199950.056.515917
TCGTATC199350.056.408879
CTGCTCG205450.055.5407755
GAAGTTT289000.054.615743
Produced by FastQC (version 0.11.5)