FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14805312
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCATC276800.019.4760462
TGAGCAT282700.019.453521
TATGAGA277100.019.44662510
GACCAAT258800.019.42961
AGGCCAC265200.019.3944641
CATATGA278000.019.3908628
ATGACAA171300.019.3519042
TGGGTGT297050.019.3400023
GCCACTC191700.019.3373323
CTGATCC177850.019.3080841
GGCCACT192200.019.3079012
CTGGGTG297350.019.2902892
TAACAGT237750.019.2842665
CCTAGAA158700.019.2741459
GCCTCTA230800.019.2687231
CTGCCTA171350.019.2582781
TTAACAG237500.019.2582514
CGTAGAA232400.019.2468782
TCGTAGA238400.019.2460561
GTAAACT231150.019.22007210