FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43274510
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG674380.15583769752678886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA376500.072.254879
GGACGTT412800.068.938219
GCACGTT304000.068.348779
GGCATTA259550.067.658689
CGACGTT296550.066.225059
TCACGTT245700.065.954729
TGGTATA286650.064.271949
GTACGTT311700.063.8243069
TAACGTT294500.063.1937189
TTACGTT270550.062.630089
AGGTATA252750.062.26549
AGACGTT317800.060.92769
ACACGTT265850.060.604019
ATACGTT276200.060.3795059
TGACGTT321750.060.15329
GTGTATA235400.059.9759949
GCGTATA216300.059.5936749
CCACGTT244500.059.047429
CTACGTT228400.059.0044029
GAGTATA188800.058.8870779
Produced by FastQC (version 0.11.5)