FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44120651
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCCC782250.021.4948395
TTTCCCT790700.021.412596
CTTTTCC806350.021.2139054
CTAGACC570800.020.88586
CCCTCAG833100.020.804229
GATTTCC398750.020.7073122
TGATTTC468850.020.5988881
TCTTTTC833250.020.5964573
GACGGCT680550.020.3934151
TAGACCT591250.020.3582447
ATTTCCA405050.020.3390463
GGCGTAC503400.020.3035099
TAAAGGC479350.020.2717175
TCCCTCA857450.020.2070068
AGACCTA613550.020.1758588
CTCTAAA502600.020.174582
ACCTAGG615350.020.11505110
AGTCTTT920750.020.0268231
GCTAGAC608050.020.009725
CTAAAGG488650.019.937684
Produced by FastQC (version 0.11.5)