FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13595914
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCAT253000.019.5468541
TATGAGA246250.019.46277410
GAGCATC250000.019.4451052
CATATGA247550.019.4252668
CTGATCC159950.019.4113791
AGGCCAC246650.019.3849961
GCCACTC177950.019.3764043
ATGACAA156550.019.3751032
GACCAAT238500.019.372181
CCGTGAA156950.019.3172236
GACCTGG156750.019.303531
GCCTACA156850.019.2988663
CCTAGAA150450.019.2942739
CTGCCTA160300.019.2878761
TCGTAGA216500.019.2847251
GGCCACT179050.019.2806262
TGGGTGT267200.019.266643
CGGCGCT179800.019.2646799
CTGGGTG267800.019.2617362
GCCTCTA214500.019.2593521
Produced by FastQC (version 0.11.5)