FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13595914
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9075256.674983381036391No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1293710.9515432357103759No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA999700.7352944421390132No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT555340.4084609537836147No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT395630.2909918376947662No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA374630.275546020664738No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG310630.22847305447798505No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC251520.1849967571139388No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC206540.15191328806581153No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT204290.1502583790983085No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT184350.13559220807074832No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG181410.13342979368654437No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC176580.12987725576963785No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT168880.12421378952529413No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC148330.10909895428876647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1015250.083.7993241
CGTTATC1022200.083.191192
TTATCCA1061200.080.0310064
GTTATCC1096050.077.5638963
TATCCAC1102800.077.339635
ATCCACC1111050.076.8036046
CACGCTG133650.074.3969651
CCACCTT1202250.070.8431248
TCCACCT1205350.070.7384957
CACCTTC1235150.069.011179
GGAAGTT242250.060.9388122
AAGTTTC244600.060.081624
TTCTGGC265350.058.442388
TTTCTGG262350.058.0091327
CTCGTAT173050.057.049358
ACGCTGC175400.056.804272
TCGTATC176000.056.093139
GCTCGTA177600.055.8749357
CTGCTCG178950.055.548415
TACGGCG4400.055.0548136
Produced by FastQC (version 0.11.5)