FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55503478
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCCC1007050.021.5357345
TTTCCCT1021850.021.3605336
CTTTTCC1038800.021.2544864
CTAGACC717500.020.8547346
GATTTCC508450.020.7765392
CCCTCAG1075650.020.7434719
TCTTTTC1073200.020.6849043
TGATTTC601200.020.675171
GACGGCT853500.020.4200441
TAGACCT740400.020.369497
ATTTCCA518250.020.3666083
GGCGTAC605800.020.2924529
AGACCTA763200.020.2682888
TAAAGGC580450.020.2489095
TCCCTCA1102400.020.2401288
CTCTAAA610050.020.1497882
ACCTAGG767700.020.1337210
AGTCTTT1190550.020.0020641
GCGTACA616600.019.93880710
TTCCCTC1102750.019.9324677
Produced by FastQC (version 0.11.5)