FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13464042
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCAT248600.019.5230391
CATATGA244550.019.4345578
GAGCATC245450.019.4214132
TATGAGA244650.019.4184410
GACCAAT232100.019.4068281
ATGACAA155550.019.3763482
TGGGTGT273600.019.374873
CTGGGTG273500.019.3674012
AGGCCAC245750.019.3668371
GCCTACA150650.019.342723
CGTAGAA209850.019.3089712
TCGTAGA214900.019.298371
CTGCCTA153800.019.2709471
GCTGCGA128800.019.268091
TGCCTAC151600.019.2611562
GACCTGG150850.019.2499451
ATATGAG247000.019.2498829
CGGCGCT180050.019.2490799
GTAAACT209600.019.22119910
GCCTCTA211250.019.2198541
Produced by FastQC (version 0.11.5)