FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13464042
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8639946.417047718656849No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1198270.8899779130219588No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA934910.6943754334693847No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT503130.3736842175626012No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT366850.2724664703214681No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA358620.26635389283545013No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG285120.21176404529932394No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC228720.16987469290425566No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC184690.1371727747135667No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT181170.1345584037839454No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT172380.12802990365003317No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG170540.12666330066409479No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC159540.11849339150902827No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT154680.11488377710051707No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC136850.10164109707916835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT951900.083.578161
CGTTATC959450.082.944192
TTATCCA1000800.079.434374
GTTATCC1035800.076.8487243
TATCCAC1040300.076.810445
ATCCACC1045850.076.561316
CACGCTG125000.073.937731
CCACCTT1133750.070.468058
TCCACCT1135750.070.430037
CACCTTC1173650.068.1375669
GGAAGTT227550.060.2214362
AAGTTTC231550.058.714354
TTCTGGC250400.057.553048
CTCGTAT161300.056.9370778
TTTCTGG251150.056.416647
GCTCGTA164600.056.0279467
TCGTATC164150.055.8449759
CTGCTCG165400.055.756955
ACGCTGC167600.055.2606622
TGCTCGT170250.054.168586
Produced by FastQC (version 0.11.5)