FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56545341
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCCC1016550.021.555085
TTTCCCT1029950.021.4081426
CTTTTCC1048400.021.2496324
CTAGACC732450.020.8839826
CCCTCAG1087300.020.7709129
GATTTCC514300.020.768462
TCTTTTC1083900.020.661593
TGATTTC608150.020.5778541
GACGGCT871200.020.4360241
ATTTCCA525650.020.295673
TCCCTCA1114000.020.2671578
GGCGTAC628500.020.2646689
TAGACCT762000.020.258177
TAAAGGC601250.020.2380035
AGACCTA784350.020.222238
ACCTAGG787350.020.13819310
CTCTAAA632400.020.0619542
AGTCTTT1200250.020.0150931
TTCCCTC1113700.019.954597
GCTAGAC780800.019.9387445
Produced by FastQC (version 0.11.5)