FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13021136
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8741256.713123954776296No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1285390.9871565737428747No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA975940.7495044979178468No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT540910.4154092238956724No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT387710.29775435875948153No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA363520.27917687058947854No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG304940.23418847633570528No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC243720.18717260921013343No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT205640.1579278489987356No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC200700.15413401718559733No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG179590.1379219140326927No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT177420.13625539277064613No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC176070.13521861687029457No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT169000.12978898308104608No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC145440.11169532366454048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT957050.083.6261441
CGTTATC960950.083.2420652
TTATCCA1001600.079.8459554
GTTATCC1033500.077.3649753
TATCCAC1038500.077.242125
ATCCACC1048300.076.601116
CACGCTG133000.074.7556841
TCCACCT1132600.070.7833257
CCACCTT1135250.070.576928
CACCTTC1166450.068.747449
GGAAGTT237000.060.0639572
AAGTTTC241050.058.7979854
TTCTGGC254000.057.4064568
ACGCTGC174400.057.177962
CTCGTAT173450.057.1138888
TTTCTGG255900.056.4820027
CTGCTCG176950.056.1763345
GCTCGTA176600.056.1673477
TCGTATC177050.055.9045689
TGCTCGT181600.054.8549086
Produced by FastQC (version 0.11.5)