FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49233481
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCCC891350.021.5311625
TTTCCCT903100.021.4130256
CTTTTCC920050.021.2660224
CTAGACC635900.020.9103156
GATTTCC448850.020.8049032
CCCTCAG954650.020.7697249
TCTTTTC949800.020.7167643
TGATTTC530650.020.5857351
TAGACCT660150.020.3295787
ATTTCCA459100.020.3119433
GACGGCT765850.020.309331
GGCGTAC554850.020.2942359
AGACCTA678750.020.260278
TCCCTCA978850.020.2494968
TAAAGGC529950.020.2233355
CTCTAAA552850.020.1850132
AGTCTTT1053200.020.0869241
ACCTAGG686350.020.02951610
GCTAGAC679650.019.960825
GCGTACA564500.019.92782410
Produced by FastQC (version 0.11.5)