FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12923625
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCAT250150.019.4986651
GAGCATC246050.019.4798032
TATGAGA244950.019.43536410
CATATGA245950.019.4295238
GACCAAT224300.019.4178371
ATGACAA152950.019.4115942
AGGCCAC233300.019.3848061
TCGTAGA207800.019.3470231
CCTCTGA153450.019.3350751
CTGGGTG255850.019.3238452
CTGCCTA149250.019.3228861
CTGATCC154300.019.3128451
CGTAGAA203450.019.3020632
TGCCTAC146950.019.2991052
GGATTAC141850.019.280171
GTAAACT203150.019.28007310
GCCTCTA208250.019.2763941
ATATGAG247700.019.2680329
TGGGTGT256400.019.2667943
GACCTGG141450.019.2568991
Produced by FastQC (version 0.11.5)