FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12923625
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8396706.497170878913618No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1196640.9259321591271799No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA919160.7112245983615278No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT497740.3851396183346391No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT359000.27778583795181305No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA347600.26896478348760505No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG281950.21816634264767046No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC226760.17546160616700035No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT184160.1424987184323284No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC182720.14138447997369158No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT170820.13217653715579025No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG169270.13097718325934093No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC158410.12257396821712176No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT151180.11697956262271614No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC133920.1036241766532223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT930600.083.734681
CGTTATC937900.083.101182
TTATCCA976900.079.7376864
GTTATCC1008650.077.316213
TATCCAC1016500.076.8785
ATCCACC1021600.076.552466
CACGCTG123550.074.596081
TCCACCT1109900.070.354977
CCACCTT1108700.070.319978
CACCTTC1144650.068.1745459
GGAAGTT220650.059.5429762
AAGTTTC222750.058.744464
TTCTGGC234350.057.1967968
CTCGTAT160350.057.1684158
ACGCTGC162500.056.7337722
TTTCTGG237750.056.200097
TCGTATC163150.056.0830889
GCTCGTA164050.055.9308437
CTGCTCG165150.055.6612435
TGCTCGT171150.053.784426