FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11315026
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTTGA107650.021.0100062
GACCTGG175800.020.9249551
GGGTCTG187600.020.851921
ATTCTAC118900.020.8447381
CAAGACC110850.020.7693926
CAGACGC120100.020.7540728
CGAGCAC100100.020.737431
CAGAATC114100.020.6996731
GACGCCT119750.020.6861310
CCATTCG156500.020.6797581
ACTTGAC102200.020.67582710
AATCAAG111450.020.6580333
TCAAGAC111850.020.6525425
TTAGTGG157200.020.6212029
GATGAAA80800.020.6125451
GAGAGTA120050.020.607047
AGACGCC118750.020.6009659
GGGCATG134850.020.596669
CAGTGAG113950.020.5812953
GTCCAAA96300.020.5719647
Produced by FastQC (version 0.11.5)