FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11315026
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9536138.42784629924845No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1674191.4796165735721685No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT982470.8682878855072891No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA588950.5205025600471443No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT414980.36675125624987515No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG414130.36600004277497905No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA371800.328589611725152No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT340830.3012189278221721No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC330810.2923634466239848No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC306110.2705340668240621No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC298920.2641796846069996No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT294640.2603971038157579No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG279000.2465747758776692No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG144820.12798910051112564No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC140660.12431257338692814No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG140020.1237469538293593No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC129200.11418444818421097No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT124120.10969484294600824No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA116100.10260692286522363No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG114660.10133427886069374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1074050.084.757151
CGTTATC1097800.082.8820952
TTATCCA1133400.080.44374
GCGGTAT13450.079.972721
GTTATCC1148900.079.3473363
TATCCAC1178600.078.3610465
ATCCACC1196100.077.587636
CGGTATC13950.077.093022
CACCTTC1255800.073.5607459
CACGCTG83250.070.8840941
TCCACCT1317400.070.240497
CCACCTT1329050.069.522468
GCGTGAT4450.067.8331451
GACGTTT11850.064.195073
AGTTTCT313750.061.6980825
GCGTTAA3900.061.0473751
TTCTGGC342700.060.615558
GAAGTTT317000.060.2209323
TTTCTGG338100.059.4919667
GGAAGTT325800.058.986432
Produced by FastQC (version 0.11.5)