FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11828246
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTGG181250.021.0913091
AAGTTGA111150.020.9918442
GGGTCTG196500.020.9380281
CGAGCAC105600.020.8870531
TCAAGAC109700.020.8768085
CCATTCG168150.020.817571
CAGAATC114800.020.7655661
GGGCATG140200.020.759959
CAGACGC129250.020.7485478
ACTTGAC106250.020.73655110
TCAGGGC140750.020.7337996
GAGCACG103900.020.7096462
ATTCTAC126750.020.7085271
CCAGACG126600.020.704987
CAAGACC110650.020.6975676
TTAAGAC105800.020.6895929
AATCAAG110650.020.6877143
ATCAAGA113650.020.6546024
CTAATGG124050.020.6183031
GACGTTA115800.020.5957431
Produced by FastQC (version 0.11.5)