FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11828246
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10684969.033427272310703No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1919021.6224045391007254No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1137520.9616979558930377No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA645410.545651485435795No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG472990.39988177452514934No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT470170.39749765096194317No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA414860.3507367026353696No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT383250.3240125374463805No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC376620.3184073107711828No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC370830.3135122485616211No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC332950.2814872128969925No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT332360.28098840690327204No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG158120.13368000631708202No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG156000.13188768647523902No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC155350.13133815444825886No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC154320.1304673575439672No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT142220.12023760750325956No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA136480.11538481698808091No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG128720.10882425001982543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1181700.084.6817251
CGTTATC1206550.082.820332
TTATCCA1247250.080.2146154
CGGTATC13600.080.0143052
GTTATCC1264800.079.115023
GCGGTAT13950.078.620531
TATCCAC1293950.078.232675
ATCCACC1309500.077.650946
CACCTTC1376750.073.548939
CACGCTG89400.073.227411
TCCACCT1443700.070.223557
CCACCTT1455350.069.565048
GCGTTAA3800.068.239631
GACGTTT12550.067.050743
GCGTGAT3850.064.040951
AGTTTCT352850.062.3668865
TTCTGGC382600.061.2494358
GAAGTTT357000.061.1776163
GGAAGTT364750.060.297282
TTTCTGG380500.060.2359927
Produced by FastQC (version 0.11.5)