FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11584967
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9966908.603304610190085No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1727591.4912342866406092No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1016390.8773352569756997No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA599260.5172738083759755No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT427440.3689609128795965No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG424270.36622460814950963No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA388950.33573682169314767No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT344280.2971782310644476No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC336170.2901777795310077No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC330470.2852576101425235No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC307010.2650072287646568No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT297720.2569882158490395No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG149840.12934003178429423No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG143190.12359983416439597No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC140960.12167492578960303No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC137680.11884367042219454No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT128490.11091097626777875No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA123440.10655187882710412No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG117860.10173529195206168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1114750.084.737511
CGTTATC1137150.082.991862
TTATCCA1177300.080.263674
GTTATCC1196700.079.029973
TATCCAC1223550.078.160575
ATCCACC1237800.077.597246
GCGGTAT13350.076.435971
CGGTATC13550.074.668592
CACCTTC1305600.073.3657769
GCGTTAA4650.073.14841
CACGCTG83200.070.7773361
TCCACCT1363150.070.249697
CCACCTT1375050.069.641748
CGTTAAC4900.069.405552
GCGTGAT3550.064.6745151
GACGTTT11250.063.841523
AGTTTCT327350.061.394015
TTCTGGC355800.059.9248858
GAAGTTT330250.059.825423
TTTCTGG352700.058.9574667
Produced by FastQC (version 0.11.5)