FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7694496
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACG70550.020.9412082
GACCTGG117550.020.9068621
CAGAATC75150.020.8892751
CCATTCG113450.020.8672921
AAGTTGA75800.020.840492
TCAAGAC77250.020.8183445
CGAGCAC73100.020.7828271
TTAAGAC71650.020.7710849
TGGTCCA75900.020.7538436
CAAGACC77600.020.75286
ATTCTAC80800.020.749231
TTAGTGG114950.020.7456879
GGGTCTG128650.020.7448061
ATCAAGA79250.020.737134
AGATCAG100350.020.7067473
GGGCATG90150.020.6814779
ACTTGAC68400.020.68056510
AGTTGAG76650.020.636883
GAGAGTA86950.020.6329787
GTCCAGT113800.020.6170318
Produced by FastQC (version 0.11.5)