FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7501062
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTGG115600.021.2299161
AAGTTGA74300.021.0679652
CGAGCAC65900.020.9323791
CAAGACC70600.020.8781556
GTCCAGT108100.020.8499688
GGGTCTG126100.020.8317491
TCAAGAC71100.020.7932195
ATTCTAC79100.020.7768961
ACAGTGA74550.020.746472
AGTTGAG75700.020.6924513
CCATTCG108250.020.669481
TTAAGAC66450.020.6589749
ATCAAGA73600.020.6551344
TGGTCCA72450.020.6486936
CCAGACG79650.020.646417
ACTTGAC64950.020.64494310
GCTAGAC86600.020.64070710
GATGAAA54050.020.6371041
AGTGAGC75000.020.6215784
AATCAAG71700.020.6194923
Produced by FastQC (version 0.11.5)