FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7501062
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6646958.861345233514935No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1177231.5694177704437051No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT668840.8916604075529572No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA396610.5287384639668357No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG286530.3819859107950314No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT284600.3794129417941086No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA255740.3409383897906723No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT236120.3147820935222239No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC220750.29429166163404596No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC219510.29263856237956704No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT201990.26928186968725226No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC200640.2674821245311664No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG99080.13208796301110431No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG97040.1293683481085745No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC92740.12363582650030087No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC92320.12307590578507417No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT86120.11481040951267966No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA81970.10927785958841563No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG78270.1043452247161802No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC75340.10043911115519374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT727500.084.722331
CGTTATC743400.082.867862
TTATCCA767400.080.494364
GTTATCC778950.079.257173
TATCCAC793850.078.486935
ATCCACC805450.077.646776
CGGTATC5250.074.4867252
GCGGTAT5300.073.7963261
CACGCTG55150.073.6174241
CACCTTC845050.073.6108559
TCCACCT892150.069.853277
CCACCTT896950.069.3894358
GCGTTAA2650.064.1707151
AGTTTCT217250.062.207045
GCGTGAT1600.061.1125831
GAAGTTT223850.060.3919143
TTCTGGC233150.060.389058
GACGTTT6000.059.497713
GGAAGTT229400.059.2749522
GCGATAT3450.059.1486631
Produced by FastQC (version 0.11.5)