FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8711428
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8008529.193119658453242No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1481091.7001690193616934No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT852340.9784159382365326No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA488390.5606313913172445No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG361610.415098420144206No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT350930.40283866204254914No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA309070.35478683862163585No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT295780.33953101604007974No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC292100.3353066799151643No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC279220.3205215034779602No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT254490.29213350555155826No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC252660.290032816663353No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC121890.13991965496357198No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG119900.13763529928732696No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG118880.13646442351357319No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC116200.13338800481390653No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT108060.12404395697238157No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA100070.11487209674464394No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG95680.10983273924780185No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC91320.10482781927371723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT881950.084.6954651
CGTTATC898800.083.0243762
TTATCCA931500.080.310464
GTTATCC945750.079.086913
TATCCAC962600.078.3785
ATCCACC973250.077.7954256
GCGGTAT6000.077.225061
CACCTTC1020150.073.843969
CACGCTG68350.072.7663041
TCCACCT1071150.070.474877
CCACCTT1080800.069.74348
CGGTATC6600.069.554122
GACGTTT8250.062.8454973
GCGTTAA1900.062.645141
AGTTTCT265750.061.9359055
GAAGTTT270500.060.864023
TTCTGGC286050.060.3187568
GGAAGTT275900.060.099012
AAGTTTC275500.059.6822784
TTTCTGG285650.059.4510467
Produced by FastQC (version 0.11.5)