FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8530783
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTTGA79800.021.1050832
GGGTCTG137850.020.9158271
GACCTGG134300.020.8707581
ATTCTAC87350.020.8425031
CAAGACC81450.020.8386346
CGAGCAC76850.020.741461
GAGCACG74650.020.7338092
ACTTGAC74850.020.7211610
TCAAGAC82650.020.6824785
AATCAAG82600.020.668853
TTAGTGG117350.020.6592859
CTAATGG91200.020.6501751
CAGAATC87500.020.6433351
TATGAAA67800.020.6368899
GATGAAA58700.020.6330781
CCAGACG91050.020.6226967
CAGACGC93300.020.62038
GAGAGTA91600.020.6069497
AGTTGAG81750.020.6011753
GGCTAGA99350.020.5825089
Produced by FastQC (version 0.11.5)