FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8530783
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7492218.782558412281734No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1341381.5723996261538948No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT767680.8998939487735181No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA455280.5336907526542405No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG331410.388487199826792No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT321000.37628433404061506No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA292600.34299313439340795No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT267800.3139219459690863No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC261870.3069706497047223No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC250060.2931266684429788No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC230830.27058477516073265No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT228430.2677714343454757No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG110420.1294371220086128No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG110080.12903856539311806No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC109550.12841728596308216No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC107470.12597905725652617No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT98730.11573380778763216No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA93500.10960306926105141No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG89440.10484383438190843No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC85430.10014321076974998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT825900.084.7268141
CGTTATC845050.082.823852
TTATCCA873200.080.3325044
GTTATCC886700.079.123823
TATCCAC901000.078.500075
ATCCACC917800.077.391916
GCGGTAT6150.076.038941
GCGTTAA2900.074.763671
CGGTATC6200.074.728212
CACCTTC961350.073.558859
CACGCTG65350.072.6000751
TCCACCT1013000.069.867037
CCACCTT1019650.069.386368
CGTTAAC3100.063.073352
AGTTTCT248150.062.337635
GAAGTTT251600.061.0267873
TTCTGGC266300.060.6901668
GACGTTT7350.060.132493
AAGTTTC257050.059.799024
TTTCTGG266500.059.4645777
Produced by FastQC (version 0.11.5)