FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15177324
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGAAA203150.021.4124182
CCCATAG180600.021.3840873
GCCCATA180650.021.349012
ATTACGA332450.021.316189
CGAGAAG178600.021.2919981
CCTTACG273100.021.2816621
CCATTAC332700.021.2671017
ATCCGAA259450.021.2640651
CCGAACA205300.021.2277093
TCTGGAA213950.021.2269331
ACACCCA209800.021.1798787
TGCCCAT188050.021.176331
TTACGAC334600.021.16934810
GACCAAT235350.021.1633261
GGACGCA238400.021.1511882
TGGAAAG205750.021.1385123
GAGAAGT173600.021.1378652
GAACACC206500.021.1256585
TCCTATA295750.021.09772310
CGGAGAT204100.021.0969832