FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15033743
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAG174050.021.398333
ATTACGA330900.021.3505049
CCTTACG280850.021.3313521
GCCCATA174250.021.3246882
CTGGAAA202350.021.24082
ATCCGAA251750.021.2306691
CCGAACA198900.021.2300153
CCATTAC332600.021.214927
TGGACGC257500.021.1925771
CGGAGAT207550.021.1859072
GGACGCA251050.021.1758062
CGAGAAG182000.021.1666031
TGCCCAT180850.021.1551321
ACACCCA205650.021.1482727
TTACGAC334150.021.13955110
CCTTCTG255550.021.1339075
ACGGAGA212100.021.1206951
GAGAAGT177300.021.106842
ACCCATG203500.021.0906419
TCCTATA286400.021.08464410
Produced by FastQC (version 0.11.5)