FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15033743
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT179343011.929364496918698No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT2125011.4134936322910403No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1219010.8108493008028673No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA929360.6181827107194795No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA703060.4676546619161974No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT662450.44064209425423867No Hit
GTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCAGC558020.3711783552505853No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC542840.3610810694316113No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG528910.3518152465423947No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC425500.28302998128942336No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC401920.2673452645824796No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT388770.2585982745614316No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT328960.21881443629839886No Hit
GTGTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCA264190.17573135313008875No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG256450.17058293466903088No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG241070.16035261478129564No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT208700.13882105075229767No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC203720.13550850244014415No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG180780.12024949475323611No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG159510.10610132154048396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1988400.084.8257751
CGTTATC2012450.083.76832
TTATCCA2097400.080.4747544
ATCCACC2176000.078.7316746
GTTATCC2146100.078.7020653
TATCCAC2168800.078.585465
GCGGTAT18250.076.731451
CACGCTG122000.076.300841
CACCTTC2325350.073.3355269
CGGTATC18850.073.273582
CCACCTT2402300.070.988468
TCCACCT2413900.070.708957
GCGTGAT7650.070.105091
GCGTTAA8050.069.265311
GGAAGTT416350.065.2357562
GACGTTT14050.063.8236433
AAGTTTC429350.062.735914
AGTTTCT437100.062.4886975
GAAGTTT441700.061.0587733
GCGTTAG2650.061.0348851
Produced by FastQC (version 0.11.5)