FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12347219
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG225600.021.4198881
CTGGAAA163450.021.4004552
ATTACGA272950.021.3972029
GAGAAGT143250.021.3591822
CGAGAAG148150.021.3438851
CCATTAC274450.021.2962847
CCCATAG144750.021.292333
TCTGGAA172150.021.2718811
CCGAACA165850.021.2429643
TTACGAC275100.021.22597310
ATCCGAA211150.021.2119461
GCCCATA145350.021.209662
TGGAAAG165600.021.1421873
ACCCATG168850.021.137779
CATTACG276550.021.1305928
GCCATTA276950.021.1040446
TGCCCAT150200.021.1038881
GACCAAT192450.021.0990891
AAGCCTA258000.021.09809510
ACACCCA170300.021.0869667
Produced by FastQC (version 0.11.5)