FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12347219
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT150633512.199791710181863No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1785851.4463580827391171No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1067730.8647534315217054No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA826180.6691223343491357No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA591440.4790066491895868No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT558920.45266873455472034No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC481500.3899663559867206No Hit
GTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCAGC464060.37584171787995335No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG447250.36222731612681364No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC362640.29370176393566844No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC351820.2849386570368599No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT332500.26929140885895037No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT284040.23004370457833462No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG227990.1846488670849687No Hit
GTGTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCA221310.1792387419385693No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG215740.17472760465332315No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT176330.14280948608751493No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC166830.13511544583440205No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG157580.12762388032479216No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG136780.11077798166534505No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA125160.101366955587327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1641900.084.807921
CGTTATC1661600.083.7414552
TTATCCA1735000.080.3652954
GTTATCC1773900.078.6436843
ATCCACC1799300.078.508966
TATCCAC1797100.078.380425
CACGCTG108250.076.1435551
GCGGTAT17000.075.6334151
CACCTTC1921250.073.310519
CGGTATC17900.071.22462
CCACCTT1998100.070.48698
TCCACCT2003150.070.3855747
GACGTTT10950.070.246753
GCGTTAA5150.067.7894741
GGAAGTT340950.065.03922
AAGTTTC349650.062.9590264
AGTTTCT357550.062.5425835
GGTATCC20750.061.851563
GAAGTTT359850.061.387023
TTCTGGC389250.060.658778
Produced by FastQC (version 0.11.5)