FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12261788
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAG145950.021.4415253
GCCCATA146200.021.396862
CCTTACG224200.021.3957981
CTGGAAA166600.021.3931712
ATTACGA270650.021.3157569
TCTGGAA174600.021.3020211
CGAGAAG151600.021.2592891
GGACGCA197550.021.245312
CCATTAC271100.021.2154587
TTACGAC272100.021.19812410
ATCCGAA206700.021.1836681
CCGAACA161450.021.1816653
TGCCCAT151750.021.1584871
AAGCCTA253600.021.14407310
GACCAAT190850.021.1262111
TGGACGC204750.021.1112461
GAGAAGT147850.021.1059572
ACCCATG162800.021.0999249
GCGTAAG186100.021.08831210
TGGAAAG169250.021.0828653