FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12261788
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT145141311.836878928260706No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1701961.388019430771434No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1021150.8327904543774529No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA795660.6488939459726428No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA566430.4619473114361462No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT533280.43491210254165213No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC460800.37580163675966344No Hit
GTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCAGC452140.36873904523549095No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG428820.3497206117085045No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC349330.28489319828396964No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC339850.2771618625277162No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT315980.2576948810401876No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT276590.2255706916479065No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG215230.17552905008633324No Hit
GTGTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCA214220.17470535292242861No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG210270.17148396302398963No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT171510.13987356493196587No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC162230.13230533752500043No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG154770.12622139609655622No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG128590.1048705131747507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1600300.084.755141
CGTTATC1619850.083.693952
TTATCCA1689500.080.409674
ATCCACC1750300.078.669996
GTTATCC1729100.078.553373
GCGGTAT15950.078.4536061
TATCCAC1751300.078.348425
CACGCTG100100.076.663371
CACCTTC1869650.073.402619
CGGTATC16950.072.212862
CCACCTT1943850.070.642258
TCCACCT1953100.070.3574457
GCGTGAT5400.069.360981
GACGTTT10800.065.7180253
GGAAGTT327800.064.489442
CGTGATC5750.064.304772
GCGTTAA5500.063.456691
AAGTTTC335950.062.1659054
AGTTTCT342550.061.9108245
GGTATCC20050.061.895663
Produced by FastQC (version 0.11.5)