FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16843968
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA367650.021.3782969
CTGGAAA227400.021.3476282
CCCATAG195350.021.3402773
GCCCATA195300.021.3215732
CCTTACG314550.021.2925681
CCATTAC370150.021.219057
TCTGGAA239800.021.2171341
TTACGAC370250.021.2162910
ATCCGAA289100.021.1827741
CCGAACA229300.021.178783
GGACGCA268750.021.1521052
CGGAGAT231600.021.1506542
ACACCCA235400.021.123837
CTGACCT197150.021.121689
TCTGACC197300.021.1111958
TCCTATA324350.021.0819910
TGCCCAT204200.021.0716611
GCCATTA371750.021.0626326
ACCCATG233850.021.06169
CGAGAAG206700.021.0565031
Produced by FastQC (version 0.11.5)