FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33659449
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTGG520500.021.160741
GGCTGGA477800.021.1051252
TGGAGAG469150.020.8643265
GAGGCCG272400.020.8089317
GTAAGCC352300.020.7385165
ATTGCAG490200.020.7135413
GCAGCTA469250.020.6979716
CTAGCCC480250.020.6906110
GATTGCA490800.020.671842
GGCCGAA287600.020.6498749
AGGCCGA288850.020.6100068
AGTGACC486000.020.5883810
AGATTGC541850.020.5730421
TTGCAGC467900.020.5604274
AGCTAGC485000.020.5219848
CTGGAGA498350.020.396594
GTGTGCG521450.020.367751
GCCGAAT292000.020.30857810
CGGCGCA256000.020.273219
AGAGTGA507850.020.2612978
Produced by FastQC (version 0.11.5)